A new simple, sensitive, precise and accurate high-performance liquid chromatography (HPLC) method of analysis for Artemether both as a bulk drug and in capsule formulations was developed and validated. The method employed mobile phase acetonitrile and phosphate buffer in the ratio 60:40 of pH 3 ±0.5. The linear regression analysis data for the calibration plots showed good linear relationship with r2 = 0.9996 in the concentration range 20-100 µg/ml. The mean value slope and intercept were 124557 and 118672 respectively. The method was validated for precision, accuracy and recovery studies. Limit of detection (LOD) and Limit of quantitation (LOQ) for Artemether were found to be 0.00045 x 10-05, 0.00013 x 10-03 respectively. The method has been successfully applied in the analysis of marketed formulations.
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